Journal: The Journal of Reproduction and Development

Article Title: Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa

doi: 10.1262/jrd.2017-141

Figure Lengend Snippet: Detection of the CD44 receptor by Western blotting (WB) in human and pig spermatozoa. In A, using a porcine-specific anti-CD44 monoclonal antibody (60224-1-Ig, Nordic BioSite, Proteintech), the CD-44 receptor was detected in pig and human spermatozoa at 85 kDa. Gel B depicts co-incubation of this monoclonal primary antibody with its specific blocking peptide (Ag7633, Nordic BioSite, Proteintech Europe, Manchester, UK), which eliminated the 85 kDa band of the full-sized CD-44 receptor in all cells/tissues. L1: ladder, L2: human, L3: non-capacitated boar spermatozoa, L4: capacitated boar spermatozoa, L5: boar seminal vesicle (somatic positive control).

Article Snippet: In A, using a porcine-specific anti-CD44 monoclonal antibody (60224-1-Ig, Nordic BioSite, Proteintech), the CD-44 receptor was detected in pig and human spermatozoa at 85 kDa.

Techniques: Western Blot, Incubation, Blocking Assay, Positive Control

Journal: The Journal of Reproduction and Development

Article Title: Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa

doi: 10.1262/jrd.2017-141

Figure Lengend Snippet: Localization of the CD44 receptor on immunocytochemistry (ICC) analysis in human (A) and pig (B: uncapacitated, C: capacitated) spermatozoa using a monoclonal antibody (60224-1-Ig, Nordic BioSite, Proteintech Europe, Manchester, UK). The insets in the top-right corner in each figure (marked A´–C´) depict ICC negative control images (where the antibody was neutralized by treatment with the specific blocking peptide CD44 fusion protein Ag7633 (Nordic BioSite, Proteintech Europe, Manchester, UK)). Human spermatozoa (positive control) had clear CD44-immunostaining over the post-acrosome area of the sperm head (Fig. 2A). In pig spermatozoa (Fig. 2B; 2C), the CD44 receptor appeared consistently localized on the plasma membrane over the post-acrosomal region, neck, and midpiece. Confocal laser scanning microscopy, scale bar: 10 μm.

Article Snippet: In A, using a porcine-specific anti-CD44 monoclonal antibody (60224-1-Ig, Nordic BioSite, Proteintech), the CD-44 receptor was detected in pig and human spermatozoa at 85 kDa.

Techniques: Immunocytochemistry, Negative Control, Blocking Assay, Positive Control, Immunostaining, Confocal Laser Scanning Microscopy

Journal: Journal of Molecular Medicine (Berlin, Germany)

Article Title: 2,8-Dihydroxyadenine disrupts epithelial integrity and alters kidney cell phenotype in vitro

doi: 10.1007/s00109-025-02625-x

Figure Lengend Snippet: Adenine phosphoribosyltransferase (APRT) and xanthine oxidoreductase (XOR) expression in kidney cell lines. a Representative confocal microscopic images of HEK293, MDCK, and HK-2 cells stained for adenine phosphoribosyltransferase (APRT, red) and xanthine oxidoreductase (XOR, green). b Representative Western blots showing APRT and XOR protein expression in HEK293, HK-2, and MDCK cells. GAPDH was used as a loading control. Bands correspond to the expected molecular weights (APRT ~ 18 kDa, XOR ~ 150 kDa). Scale bar, 50 µm

Article Snippet: Antibodies used for immunocytochemistry studies were APRT (21,405–1-AP, Proteintech, Manchester, UK), XOR (55,156–1-AP, Proteintech, Manchester, UK) and CD44 (60224–1-Ig, Proteintech, Manchester, UK).

Techniques: Expressing, Staining, Western Blot, Control

Journal: Journal of Musculoskeletal & Neuronal Interactions

Article Title: Characterization and Biocompatibility Assessment of 3D-Printed HA/PCL Porous Bionic Bone Scaffold: in Vitro and in Vivo Evaluation

doi: 10.22540/JMNI-25-119

Figure Lengend Snippet: The identification of VEPCs using CD31 immunofluorescence staining (a–b: 100X; c–d: 200X). DAPI was used for nucleus staining (blue), and CD31 for VEPCs staining (red). VEPCs, vascular endothelial progenitor cells; DAPI, 4’,6-diamidino-2-phenylindole.

Article Snippet: The primary antibodies used were CD44 (60224-1-1g, 1:100, Proteintech) and CD31 (66065-2-1g, 1:100, Proteintech).

Techniques: Immunofluorescence, Staining